The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1974, 113: 18-26.
Copyright © 1974 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lehrer, S. B.
Right arrow Articles by Vaughan, J. H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Lehrer, S. B.
Right arrow Articles by Vaughan, J. H.

Extraction and Partial Purification of the Histamine-Sensitizing Factor of Bordetella Pertussis1,2,

Samuel B. Lehrer, Eng M. Tan and John H. Vaughan

From the Division of Allergy and Immunology and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

The histamine-sensitizing factor (HSF) of Bordetella pertussis has been recovered in soluble form by treatment of bacterial cells with 4 M urea, 1 M NaCl buffered with 0.1 M sodium phosphate at pH 6.4. Besides histamine-sensitizing activity, this extract has leukocytosis-promoting activity and is a potent adjuvant for hemagglutinating and reaginic antibodies. Through extraction and gel filtration column chromatography, a 280-fold purification of HSF has been achieved resulting in a purified preparation with a 50% sensitizing dose (SD50) of about 60 ng. Chemical analysis of this purified extract demonstrated protein and lipid to be the major components. Since other pertussis biologic activity such as leukocytosis-promoting and adjuvant activities in addition to histamine-sensitizing activity were still present in this highly purified extract of HSF, it is possible that one molecular species might be responsible for all these activities.

Footnotes

1 This is publication No. 770 from the Department of Experimental Pathology. This work was supported by United States Public Health Service Grants AI 10386 and AM 12198.

2 This work was presented in part at the meetings of the Federation of American Societies for Experimental Biology, April, 1973.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.