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The IgM Antibody Site

I. Increase of Binding Affinity of Rat 19S and 7S anti-Dinitrophenyl Antibodies During the Course of the Immune Response

Institut d'Immunobiologie, INSERM and CNRS, Hôpital Broussais, 96, Rue Didot, 75674 Paris, Cedex 14, France

Abstract

Rats immunized with DNP-Salmonella and DNP-BGG were bled at different times ranging from 5 days to 8 months. The 19S and 7S antibody fractions were separated by gel filtration and their binding affinities were measured with tritiated -DNP-Lys. Five days after the first injection, both 7S and 19S fractions were heterogeneous and their average K₀ was low. The binding affinity of the 7S fraction increased progressively with time. The 19S fraction showed a different pattern of evolution. Two restricted populations of sites evolved from the starting heterogeneous, but normally distributed family. The K₀ of the high affinity population of 19S antibodies followed the same maturation process as the corresponding 7S antibodies, whereas the K₀ of the low affinity population of 19S antibodies remained fairly constant throughout the whole experiment.