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Distribution and Mobility of 2-Microglobulin on the Human Lymphocyte Membrane: Immunofluorescence and Immunoferritin Studies¹

Laboratorie d'Immunologie des Tumeurs, Institut de Recherches sur les Maladies du Sang, Centre Hayem, Hôpital Saint Louis, 75010 Paris, France and Service d'Immuno-chimie, Institut Pasteur, Lyon, France

Abstract

2-Microglobulin (2-µ) was detected on the surface of human peripheral blood cells by direct or indirect immunofluorescence methods and by indirect immunoferritin labeling in electron microscopy with specific anti-2-µ sera and conjugates. 2-µ was expressed, although not with the same intensity on the membrane of all lymphocytes, including B and T derived cells, on polymorphonuclear leukocytes, but not on erythrocytes. The antibody-induced redistribution and capping of 2-µ on the membrane of living lymphocytes could not be obtained at 37°C by bivalent anti-2-µ antibodies alone, but was readily provoked upon addition of anti-Ig sera to anti-2-µ antibody-coated cells. The technical conditions for the redistribution of all detectable 2-µ were defined by double immunofluorescence techniques. In such conditions, 2-µ migrates independently of surface Ig on the membrane of B lymphocytes, indicating that these molecules are independent on the cell surface.

Footnotes

¹ This work was supported by contracts from DGRST and INSERM.

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