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The Journal of Immunology, 1974, 112: 2013-2019.
Copyright © 1974 by The American Association of Immunologists, Inc.
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In Vitro Stimulation of Rabbit Lymphocytes after Immunization with Live and Inactivated Rabies Vaccines1

Tadeusz J. Wiktor, Isao Kamo2 and Hilary Koprowski

Wistar Institute of Anatomy and Biology, World Health Organization International Reference Center for Rabies, 36th Street at Spruce, Philadelphia, Pennsylvania 19104

Abstract

Spleen lymphocytes from rabbits immunized with live or inactivated rabies virus were exposed in vitro to rabies antigens. Blastogenesis of sensitized spleen cells was measured by incorporation of 3H thymidine into the DNA of actively proliferating cells. The maximum stimulation ratio by rabies antigens was observed 8 days after immunization and animals remained sensitized for at least 175 days. No differences were noted when either live or inactivated viruses were used for immunization or for stimulation of spleen lymphocytes. Purified rabies virions, "soluble antigens," and glycoprotein fraction showed a stimulating effect but not the nucleocapsid fraction of rabies virions.

Footnotes

1 This investigation was supported in part by United States Public Health Service Research Grants AI-09706 from the National Institute of Allergy and Infectious Diseases, RR-05540 from the Division of Research Resources, Contract NIH-71-2292 with the National Institutes of Health, and funds from the world Health Organization.

2 Visiting scientist from Department of Bacteriology, Tohoku University School of Medicine, Sendai, Japan. Present address: Albert Einstein Medical Center, Tabor and Old York Roads, Philadelphia, Pennsylvania 19141.






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