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Studies on Antibody Induction in Vitro

III. Cellular Requirements for the Induction of Antibody Synthesis by Solubilized T2 Phage and Immunogenic RNA¹

Department of Immunology, The Public Health Research Institute of The City of New York, Inc., New York, New York 10016

Abstract

The elimination of cells with specific receptors for the solubilized T2 antigen (S-T2) was accomplished by passage of spleen cells from unimmunized rabbits through antigen-coated glass bead columns. This procedure resulted in a population of lymphoid cells incapable of antibody-production in response to S-T2 in vitro. Such a population, devoid of antigen-specific cells, was tested for its ability to produce antibody in response to two fractions of RNA extracted from peritoneal exudate cells exposed to the T2 phage and then fractionated on methylated BSA Kieselguhr columns. The unresponsiveness of the cells filtered through antigencoated glass beads to S-T2 extends to the MAK III fraction containing RNA-antigen complexes, but not the MAK II fraction containing informational RNA. Therefore, the response to the informational RNA is independent of cells possessing specific antigen recognition.

Footnotes

¹ This work was supported in part by Grant GB-29018X from the National Science Foundation, and Training Grant AI-00408 from the National Institutes of Health.