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The Journal of Immunology, 1974, 112: 1971-1980.
Copyright © 1974 by The American Association of Immunologists, Inc.
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Evidence for T Lymphocyte Subpopulations in the Rabbit1

Michael W. Fanger2, Andy C. Reese, Melvin D. Schoenberg, Abram B. Stavitsky and Alice L. Reese

Departments of Microbiology and Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Abstract

A goat antibody to rabbit thymus cells (ATG) was used to study T lymphocyte distributions and subpopulations in the rabbit. ATG inhibited transformation of peripheral blood lymphocytes stimulated by phytohemagglutinin (PHA) and concanavalin A (Con A) but not by goat anti-rabbit Fab. The distribution of T lymphocytes was determined by the ability of lymphocytes from the various tissues to absorb this inhibitory activity. Lymph node, peripheral blood, and thymus lymphocytes, in that order, were most capable of absorbing the ATG activity. Fluorescein-conjugated ATG caused fluorescence of 100% of thymus cells but lower percentages of lymphocytes from other organs. In addition, the intensity of staining by fluorescent antibody varied among lymphocytes even from the same organ. The results indicate the presence of rabbit T cell subpopulations which differ in the quantity of T antigen per cell. The effect of ATG on mitogen stimulation of lymphocytes from the various lymphoid organs indicated that the T cell subsets differed in their response to PHA and Con A, and in their susceptibility to the inhibitory effect of ATG. Evidence is also presented which suggests the presence of lymphocytes which possess both surface T antigen and Ig determinants.

Footnotes

1 This work was supported by Research Grants AI08788, AI10148, and AI01865 from the United States Public Health Service and Training Grant GM00171.

2 Career Development Awardee, AI25732.






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