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Periodate-Induced Lymphocyte Transformation

IV. Mitogenic Effect of NaIO₄ Treated Lymphocytes Upon Autologous Lymphocytes¹

From the Department of Pathology, University of Southern California School of Medicine, Los Angeles, California 90033

Abstract

If lymphocytes are inactivated with mitomycin C, ⁶⁰Co irradiation, or ultraviolet (UV) irradiation, and treated with sodium periodate (NaIO₄), they are capable of inducing transformation of autologous lymphocytes in vitro. The optimal ratio of IO₄^--treated stimulator cells to untreated autologous lymphocytes is 1:2 or 1:4. Maximum incorporation of tritiated thymidine (³H-TdR) in autologous mixed lymphocyte cultures (A-MLC) occurs after 3 days. As with direct NaIO₄ transformation of lymphocytes, the transformation in the A-MLC can be blocked by sodium borohydride (NaBH₄). Overoxidation of the cells by NaIO₄ also diminishes their ability to induce an autologous mixed lymphocyte reaction (A-MLR). Incubation of the stimulator cells after treatment with NaIO₄ results in a progressively declining ability to induce transformation in the responder cells. We failed to find any evidence of a blastogenic factor released into the medium by the treated cells during periods ranging from 2 hr to 72 hr after NaIO₄ treatment. NaIO₄ treatment of autologous red blood cells does not render them capable of inducing transformation of autologous lymphocytes.

Footnotes

¹ This investigation was supported by The John A. Hartford Foundation, Inc., the Council for Tobacco Research—United States of America, The Robert E. and May E. Wright Estate, the Richard King Mellon Charitable Trusts, and the Professional Staff Association of the Los Angeles County—University of Southern California Medical Center.