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The Journal of Immunology, 1974, 112: 1861-1866.
Copyright © 1974 by The American Association of Immunologists, Inc.
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Migration Inhibition Factor (MIF) and Migration Stimulation Factor (MSF) in Fetal Calf Serum1

Roy A. Fox2, Douglas S. Gregory and Joseph D. Feldman

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

Non-heat-inactivated fetal calf serum contains a factor, FCS-MIF, that inhibits in vitro the migration of guinea pig peritoneal macrophages. FCS-MIF is partially or completely inactivated by dialysis, repeated freezing and thawing, heating to 56°C for 30 min, and changes of pH beyond the range of 6.0 to 7.7. Chromatography in different gels and with different buffers suggests that several fractions may have MIF activity.

FCS also contains a factor, FCS-MSF, that stimulates the migration of guinea pig macrophages. It is detected after MIF is removed by chromatography on DEAE-Sephadex A-25. A stimulatory factor is also present in supernatants of sensitized guinea pig lymphoid cells stimulated with antigen and is detected when the supernatants are treated in the same fashion as FCS to uncover FCS-MSF.

Footnotes

1 This is Publication 768 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037. The work was supported by United States Public Health Service Grant AI-07007 and Atomic Energy Commission Contract AT(04-3)-779.

2 During part of these studies Roy A. Fox was supported by a Medical Research Council (United Kingdom) Travelling Scholarship. Present address: Department of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.






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