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Clonal Nature of the Immune Response to Phosphorylcholine

II. Idiotypic Specificity and Binding Characteristics of anti-Phosphorylcholine Antibodies¹

From the Departments of Pathology and Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110 and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

The clonal nature of the immune response to phosphorylcholine (PC) was studied at the level of the antibody-producing cell (PFC) with the highly sensitive parameters of idiotypes and binding characteristics as specific markers. As a basis for comparison, the characteristics of the anti-PC IgM antibody produced in different inbred strains of mice were related in PFC inhibition assays with those exhibited by four different PC-binding myeloma proteins, HOPC 8, M^cPC 603, MOPC 167, and MOPC 511. Each of these myelomas possesses distinctly different idiotypic determinants and binding affinities for PC, glycerophosphorylcholine (GPC) and choline (C). The induced anti-PC antibody in each of the strains tested was solely of the IgM class and bound PC ; GPC ; C. In BALB/c and A, but not CBA, no affinity maturation occurred during the immune response which indicates a restriction in the affinity heterogeneity. As determined from the PFC inhibition profiles, BALB/c and A strain anti-PC antibody had the same binding characteristics as HOPC 8 and that of BALB/c possessed the idiotypic determinants of this myeloma protein. As shown in a previous paper, the receptors on PC-specific antigen-binding cells (ABC) possessed the identical characteristics of class, specificity, and idiotypes as shown by the secreted antibody. These results, therefore, provide evidence for a direct link between the antibody secreted by PFC and the receptor molecules on ABC.

Footnotes

¹ This work was supported in part by Brown & Williamson Tobacco Corporation; Larus & Brother Company, Inc.; Liggett & Myers Incorporated; Lorillard, a Division of Loews Theatres, Inc.; Philip Morris Incorporated; R. J. Reynolds Tobacco Company; United States Tobacco Company; and Tobacco Associates, Inc.

² Departments of Pathology and Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.

³ Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014.

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