HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Götze, D. Articles by Reisfeld, R. A. Search for Related Content PubMed Articles by Götze, D. Articles by Reisfeld, R. A.

Immunogenicity and Partial Purification of Soluble H-2 Antigens Extracted with Hypertonic Salt¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

H-2^d antigen was solubilized by 3 M KCl extraction from cultured murine lymphoid cells L1210, derived from DBA/2 mice. Yields of protein and specific inhibitory activity were found to be optimal when the cells were harvested during log growth. A 5- to 7-fold increase in specific H-2^d antigen activity was achieved by Sephadex gel filtration and cellulose ion exchange chromatography. Antibodies against H-2K^d and H-2D^d determinants were produced by immunization experiments within the allogeneic system. Immunization with soluble H-2^d antigen led to specific sensitization with respect to cellular immunity, indicating that the soluble material maintained its integrity as a serologically defined histocompatibility antigen.

Footnotes

¹ This is Publication 669 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California. This work was supported by United States Public Health Service Grant AI 07007.

² D. G. is a Visiting Scientist supported by the Deutsche Forschungsgemeinschaft, SFB 37 (University of Munich), Germany.