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Studies of Cellular Immunity in Chagas Disease: Effect of Glutaraldehyde-Treated Specific Antigen on Inhibition of Leukocyte Migration¹

From the Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina

Abstract

Delayed hypersensitivity was studied in chronic protozoal infection produced by the Trypanosoma cruzi (Chagas disease). Blood peripheral leukocyte migration inhibition was selected for studying cellular immune response in vitro. Two kinds of antigen preparations were used: a soluble extract of epimastigotes (SAg), and a modification of the antigen after its treatment with glutaraldehyde (MAg). When SAg was added to the medium no significant inhibition of migration was obtained in spite of using a concentration of antigen as high as 200 µg/ml. Similar results were obtained with normal cells. When the MAg was used, specific inhibition was obtained with chronic Chagas leukocytes. The inhibition was significant with a concentration of MAg as low as 5 µg/ml (p < 0.001).

Footnotes

¹ This work was supported by Comisión para el estudio de la enfermedad de Chagas, Universidad de Buenos Aires and Research Grant 3817, Consejo de Investigaciones Cientificas y Técnicas, Argentina.

² Career investigator of Consejo Nacional de Investigaciones Cientificas y Técnicas, Argentina.