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Early Detection of Lymphocyte Stimulation and Mixed Lymphocyte Interaction in Man with a Semimicro Protein Synthesis Assay

From the Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Abstract

A rapid semimicro assay for lymphocyte activation in man is described. It is based upon the stimulation of protein synthesis (SPS) in peripheral blood lymphocytes cultured in serum-free medium with soluble antigens, phytomitogens or allogeneic lymphocytes. Cellular response to PHA is discernible in 4 hr and response to antigen in 16 to 24 hr. Stimulation in mixed lymphocyte cultures can be detected as early as 8 to 10 hr and invariably by 42 hr. The assay permits detection of lymphocyte activation earlier and with greater precision than the widely employed thymidine incorporation blast transformation system.

Footnotes

¹ Present address: Department of Medicine, Johns Hopkins University School of Medicine, Good Samaritan Hospital, 5601 Loch Raven Boulevard, Baltimore, Maryland 21239.

² Present address: Department of Surgery, Peter Bent Brigham Hospital, Boston, Massachusetts 02115.