HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Grubb, A. O. Search for Related Content PubMed Articles by Grubb, A. O.

Crossed Immunoelectrophoresis and Electroimmunoassay of IgM¹

From the Department of Clinical Chemistry, University of Lund, Malmö General Hospital, S-214 01 Malmö, Sweden

Abstract

Analysis of IgM by crossed immunoelectrophoresis is possible if the original technique is modified by the use of: agarose gel with little, or preferably no, electroendosmosis; a high pH of 10.3; and an antibody population essentially isoelectric at pH 10.3. Such an antibody population can easily be produced by a chemical modification, under mild conditions, of about one-third of the carboxylic acid groups of the antibodies. This analytical system also permits rapid quantitation of IgM by the electroimmunoassay.

Footnotes

¹ This work was supported by the Medical Faculty, University of Lund and by the Swedish Medical Research Council (Project B74-13X-581-10C).