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Attempts to Induce Cultures of BALB/c Myeloma and P3-J Burkitt's Lymphoma Cells to Produce Specific Phage Neutralizing Antibody¹

From the Department of Microbiology, University of Washington, Seattle, Washington 98195 and Department of Microbiology, Mayo Medical School, Mayo Foundation, Rochester, Minnesota 55901

Abstract

An attempt was made to determine if the P3-J line of Burkitt's lymphoma cells could synthesize specific antibody in response after exposure to bacteriophage as reported by Kamei and Moore (1, 2). The cells were exposed to various concentrations of phages MS-2 or T₂ and cultured for up to 10 days, but we were unable to detect significant neutralizing activity in any experimental situation. We also attempted to induce a BALB/c myeloma tumor with specific antibody activity for phage MS-2. While two in vivo tumors had characteristics of producing neutralizing antibody for MS-2 on adaptation of one of these tumors to growth in cell culture, no activity could be detected. Further, exposure of the cultured tumor cells to MS-2 did not result in the synthesis of neutralizing antibody.

Footnotes

¹ This research was supported by United States Public Health Service Grants AI-08044 from the Institute of Allergy and Infectious Diseases and CA-13333 and CA-12505 from the National Cancer Institute.

² Requests for reprints should be addressed to Dr. Robert G. Krueger, Department of Microbiology, Mayo Medical School, Rochester, Minnesota 55901.