HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hierholzer, J. C. Articles by Barme, M. Search for Related Content PubMed Articles by Hierholzer, J. C. Articles by Barme, M.

Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method

Respiratory Virology Section, Center for Disease Control, Public Health Service, United States Department of Health, Education and Welfare, Atlanta, Georgia 30333 and the Department of Virology, Pasteur Institute, Paris 15^e, France

Abstract

The complex antigenic properties of adenovirus-soluble complete and incomplete hemagglutinins were utilized to study the specificity of adenovirus anti-hemagglutinin sera in immunodiffusion and counterimmunoelectrophoresis tests. Pentons, penton dimers, fiber dimers, and/or dodecons of adenovirus types 2, 3, 5, 7a, 8, and 11 were extensively purified by ion-exchange and exclusion chromatographies. Components were determined to be purified proteins by immunologic, electron microscopic, and polyacrylamide gel electrophoretic criteria. Specific antigenic components were identified by immunoelectrophoresis and by various biologic characteristics. Rabbit antisera prepared against these hemagglutinating components were type-specific in standard serologic, Ouchterlony immunodiffusion, immunoelectrophoretic, and counterimmunoelectrophoretic tests. Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes.

Footnotes

¹ Respiratory Virology Section, Center for Disease Control, Public Health Service, United States Department of Health, Education, and Welfare, Atlanta, Georgia 30333.

² Department of Virology, Pasteur Institute, Paris 15^e, France.

This article has been cited by other articles:

				M. Arens Methods for Subtyping and Molecular Comparison of Human Viral Genomes Clin. Microbiol. Rev., October 1, 1999; 12(4): 612 - 626. [Abstract] [Full Text] [PDF]