HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ishizaka, T. Articles by Ishizaka, K. Search for Related Content PubMed Articles by Ishizaka, T. Articles by Ishizaka, K.

Mechanisms of Passive Sensitization

IV. Dissociation of IgE Molecules from Basophil Receptors at Acid PH¹

Department of Medicine and Microbiology, The Johns Hopkins University, and The O'Neill Laboratories of The Good Samaritan Hospital, Baltimore, Maryland 21239

Abstract

The mononuclear cell fraction of human leukocytes was exposed to different pH at 0°C and the presence of cell-bound IgE molecules on basophil granulocytes was examined by autoradiography. The results showed that both cell-bound normal IgE and E-myeloma protein bound to the basophils by passive sensitization dissociated from the cells upon exposure to pH 4 or lower. The receptor molecules on basophils was not degraded by the treatment. Basophils exposed to acid pH combined more IgE than untreated cells upon incubation with E myeloma protein at neutral pH. The results indicated that the binding between IgE and receptor molecules was reversible and did not involve a covalent bond.

Footnotes

¹ The work was supported by Research Grant AI-10060 from the United States Public Health Service. This paper is Publication 104 from the O'Neill Laboratories at the Good Samaritan Hospital.

This article has been cited by other articles:

				R. Hamburger Peptide inhibition of the Prausnitz-Kustner reaction Science, August 1, 1975; 189(4200): 389 - 390. [Abstract] [PDF]