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Failure of Terminal Complement Components to Induce Lysis of Moloney Virus Transformed Lymphocytes¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92307

Abstract

Antibody-dependent complement-mediated lysis of a viral-induced lymphoma cell was confined to the G₁ phase of the cell cycle. During the portion of the cell cycle in which the cells were resistant to complement-mediated lysis (S, G₂, and M), C5 and C8 became bound to the plasma membrane and ultrastructural lesions characteristic of complement membrane attack developed after incubation of the cells with antibody and complement. Quantitative studies revealed that the plasma membrane of cells bound equivalent numbers of C8 molecules in all phases of the cell cycle. These findings emphasize the importance of events occurring subsequent to activation and attachment of the cytolytic effector system for destruction of this tumor cell.

Footnotes

¹ This is Publication 714 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California. This research was supported by United States Public Health Service Grants AI-07007, CA 14692, and AI-09484, the Violet June Kertell Memorial Grant for Research on Multiple Sclerosis (MS-656 A) and by Contract NIH-NCI-E-73-3204 within the Virus Cancer Program of the National Cancer Institute.

² Drs. Cooper and Oldstone are recipients of United States Public Health Service Research Career Development Awards KO4 33630 and KO4 42580, respectively.

³ Dr. Polley was supported by a Dernham Fellowship of the American Cancer Society, California Division (No. D-133). Dr. Polley is presently affiliated with the Department of Medicine at the New York Hospital, Cornell Medical Center, New York, New York.

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