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Antibody-Mediated Target Cell Lysis by Nonimmune Cells: The Use of Anti-Immunoglobulin to Distinguish Effector Cell Populations¹

From the Max-Planck-Institut für Immunbiologie, Freiburg, Germany and the Department of Immunology, Research Institute, The Hospital for Sick Children, Toronto, Canada

Abstract

The role of a goat IgG anti-rabbit immunoglobulin on antibody-mediated target cell lysis by nonimmune cells was investigated. Using a homologous system of rabbit effector cells and rabbit anti-fowl erythrocyte serum, two populations of cells effective in the lysis of antibody-coated target cells could be differentiated by anti-immunoglobulin. In the presence of the anti-immunoglobulin, the cytotoxicity caused by phagocytic cells was suppressed. The IgG or (Fab')₂ fraction of the anti-immunoglobulin enhanced target cell destruction initiated by purified lymph node and spleen lymphocytes and to a lesser extent that initiated by purified thymus cells. Monovalent (Fab) anti-immunoglobulin decreased the cytotoxic activity of all effector cells. The mechanism of enhancement appeared to be due to cross-linking between effector lymphocytes and antibody-coated target cells by the divalent anti-immunoglobulin.

Footnotes

¹ Supported by grants from the Deutsche Forschungsgemeinschaft and the Medical Research Council of Canada (MA4875).

² Address all correspondence to: Dr. Klaus Resch, Max-Planck-Institut für Immunbiologie, D-78 Freiburg i. Br., Stübeweg 51, West Germany.