HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gormus, B. J. Articles by Shands, J. W. Search for Related Content PubMed Articles by Gormus, B. J. Articles by Shands, J. W., Jr.

Endotoxin-Stimulated Spleen Cells: Mitogenesis, the Occurrence of the C3 Receptor, and the Production of Immunoglobulin¹

From the Department of Immunology and Medical Microbiology, University of Florida, College of Medicine, Gainesville, Florida 32610

Abstract

Endotoxin (LPS)-stimulated mouse spleen cells were investigated in an attempt to correlate three markers for B lymphocytes: 1) LPS-induced blastogenesis, 2) rosette formation via the C3 receptor site, and 3) immunoglobulin production. After 24 hr stimulation 86% of LPS-induced blast cells formed rosettes indicating that these cells were B lymphocytes. Subsequently there was a progressive decrease in the percentage of rosette-forming blast cells to a low of 39% at 72 hr. The percentage of cells producing IgM increased progressively to a maximum of 54% after 72 hr of LPS stimulation These data indicate that at given times correlations between the three markers vary, and that no one of the three alone allows a strict categorization of cell types. The data presented are consistent with the hypothesis that LPS is a mitogen for B lymphocytes, although the possible involvement of other cell types was not ruled out.

Footnotes

¹ This work was supported in part by Public Health Service Grants AI-07257, AI-05345, AI-03212 and 5T-IAI-0128 from National Institutes of Allergy and Infectious Diseases.