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The Journal of Immunology, 1974, 112: 675-682.
Copyright © 1974 by The American Association of Immunologists, Inc.
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Similarity of Migration Inhibitory Factor(s) Produced by Human Lymphoid Cell Line and Phytohemagglutinin and Tuberculin-Stimulated Human Peripheral Lymphocytes1

Photini S. Papageorgiou, Carol F. Sorokin and Philip R. Glade2

From the Department of Pediatrics, Division of Infectious Diseases, Mount Sinai School of Medicine of the City University of New York, New York, New York 10029

Abstract

The migration inhibitory factor(s) (MIF) derived from the supernatants of an established human lymphoid cell line (PGLC-33H) and from human peripheral lymphocytes stimulated in vitro by phytohemagglutinin (PHA) and tuberculin (PPD) were characterized and compared. Separation of these supernatants by differential ultrafiltration showed peak inhibitory activity for all three in the fraction containing molecules of the size 10,000 to 50,000. Separation by Sephadex gel filtration (G-75) revealed similar elution profiles for these MIF. Peak inhibitory activity eluted between enzyme markers of m.w. 17,000 and 25,000. The MIF activity contained in these supernatants was found to be heat stable (56°C for 30 min) and chymotrypsin sensitive. The MIF derived from human long term lymphoid cell lines appears to be similar to the MIF produced by human peripheral lymphocytes stimulated in vitro by mitogen and antigen.

Footnotes

1 Presented in part at the 57th Annual Meeting of the Federation of American Societies for Experimental Biology, April 1973. Supported by Research Grant No. 1RO1A110422, Training Grant A100445 and Genetic Center Grant GM19443 from the National Institutes of Health, United States Public Health Service.

2 Dr. P. R. Glade is recipient of a Research Career Development Award (A1-46371) of the United States Public Health Service.






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