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In Vitro Anamnestic Response of Rabbit Lymph Node Cells: Evidence for Cell Collaboration in Induction and Regulation¹

From the Department of Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Abstract

An in vitro model and a highly specific goat IgG antibody to rabbit thymocytes (ATG) were utilized to determine whether thymus-dependent (T) lymphocytes participated in the anamnestic antibody response. The addition of various amounts of keyhole limpet hemocyanin (KLH) to lymph node cells, primed in vivo with KLH either 6 days or 2 months previously, induced the early synthesis of DNA and RNA, followed by the synthesis of IgM and/or IgG antibody to KLH. This antibody response was highly antigen concentration-dependent: the addition of 10 or 100 µg of KLH induced much less antibody synthesis than 1 µg. When ATG was present in cultures with KLH, the induction of nucleic acid and antibody syntheses was markedly inhibited. Thus it appears that these antigen-induced syntheses are mediated by T lymphocytes, and that in the rabbit the collaboration of T and bursal-equivalent (B) lymphocytes is required for induction of the anamnestic response to KLH. Kinetic analysis indicated that the KLH-induced events, mediated by T lymphocytes and prerequisite for the antibody response, occurred during the 1st day of culture. Evidence was also obtained that T lymphocytes were involved in the nonspecific regulation (enhancement) by normal goat globulin of the antibody response to KLH. The relation of the mechanisms of specific induction to those of regulation of antibody synthesis and the question of how ATG affects these mechanisms were discussed.

Footnotes

¹ Supported by Research Grant AI-1865 and Training Grant 5T1-GM-171 from the United States Public Health Service.