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Activation of Rat Mast Cells by Low Molecular Weight Stimuli¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

Radiolabeled mast cell activators, compound 48/80, and a cationic protein from neutrophils were shown to bind almost exclusively to mast cells in suspensions of rat peritoneal cells. A correlation was obtained between the binding of activator and release of ³H-serotonin at low concentrations of activator. It was shown that activator binding was substantially enhanced by the release process itself, thus demonstrating that release of amines exposes new sites for binding of activator. Saturation binding studies using compound 48/80 indicated that approximately 6x10¹⁰ molecules of activator could be bound per mast cell under conditions in which release of vasoactive amines occurs. A third mast cell activator, polymyxin B, was shown to compete with compound 48/80 for binding to mast cells.

A technique has also been described for determining accurately the release of amines from mast cells. ³H-serotonin was incorporated in mast cells in vitro and specifically released by agents which stimulate the cells.

Footnotes

¹ This is publication 745 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, California. This work was supported by the United States Public Health Service Grant AI-07007, the American Heart Association, the Council for Tobacco Research, and GMS Grant GM19322-02.

² Supported by United States Public Health Service Training Grant 5-T01-GM00683-13.

³ Recipient of United States Public Health Service Career Development Award GM42567-04.

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