HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gorczynski, R. M. Articles by Rittenberg, M. B. Search for Related Content PubMed Articles by Gorczynski, R. M. Articles by Rittenberg, M. B.

Stimulation of Early Protein Synthesis as an Assay of Immune Reactivity: Analysis of the Cells Responding to Mitogens and Alloantigens¹

From the Imperial Cancer Research Fund, Tumour Immunology Unit, Department of Zoology, University College London, London WC1E 6BT, England

Abstract

Spleen cells from nonimmune mice were cultured for 24 hr in the presence of phytohemagglutinin (PHA), lipopolysaccharide (LPS), or alloantigens. Data are presented to show that within this time period all three stimuli caused an increase in protein synthesis. The response to PHA and alloantigens was defined as a T cell response by virtue of the effects of anti- antibody, adult thymectomy, and velocity cell sedimentation. Similarly, the response to LPS was determined to be a B cell response since it was unaffected by anti- antibody or adult thymectomy but was abolished by treatment of responder cells with anti-Ig antiserum and complement. Furthermore, the cells responding to LPS were physically separable from those responding to PHA and alloantigens and sedimented with a velocity characteristic of B lymphocytes.

Footnotes

¹ Supported by an Imperial Cancer Research Fund grant to Professor N. A. Mitchison and by United States Public Health Service Grants (to M. B. R.) AM 13173 and CA 12355 from the National Cancer Institute.

² Send all correspondence to: Dr. R. M. Gorczynski, Tumour Immunology Unit, Department of Zoology, University College London, Gower Street, London WC1E 6BT, England.

³ Present Address: Dr. M. B. Rittenberg, Division of Immunology and Allergy, University of Oregon Medical School, 3181 S.W. Sam Jackson Park Road, Portland, Oregon 97201.