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IgM Anamnestic Immune Response to the Haptenic Determinant DNP on a Thymus-Independent Carrier

From the Institut of Immunobiology, Hôpital Broussais, 75674 Paris Cedex 14

Abstract

Antibody formation in mice against a haptenic determinant such as dinitrophenyl (DNP),³ is strictly related to the immunogenicity and nature of the carrier. Thus, a carrier that interacts only with non-thymus-derived (B) lymphocytes, e.g., levan (LE) (1), induces in vivo a thymus-independent primary response to DNP (2). The DNP-LE conjugate produces only IgM antibody against DNP (2), and the response is not potentiated by Freund's adjuvants (3). This report describes the anamnestic response to the DNP-LE conjugate.

LE with the reduced molecular weight of 100,000 was coupled to DNP, as previously described (2). Male CBA mice (20 to 25 g) were injected i.v. with different doses of DNP₆-LE. Sixteen weeks later mice were challenged i.v. with 10 µg of DNP₆-LE. In transfer experiments CBA mice were primed i.v. with 100 µg of DNP₆-LE, and 1 or 4 weeks later their spleens were removed and homogenized in medium 199 (4).

Footnotes

³ Abbreviations used in this paper: DNP, dinitrophenyl; B, non-thymus derived; LE, levan; 2-ME, 2-mercaptoethanol.

¹ Seconded from INSERM.

² Seconded from College de France.