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Autoantibodies in NZB Mice Detected by Lysis of DBA/2 Lymphoma Cells¹

From the Departments of Microbiology and Medicine, School of Medicine, State University of New York at Buffalo, Buffalo, New York 14214

Abstract

Natural antibodies lytic for DBA/2 lymphoma cells, L5178Y, were shown in NZB mice by means of plaque assay demonstrating antibody-forming cells and spot test detecting serum antibodies. The antibodies were of the IgM class, resisted heating to 56°C for 30 min, and showed optimal binding activity at 4°C. They were efficiently removed by murine kidney and spleen cells and less so by thymocytes, brain, and liver cells. Since syngenic cells removed them as readily as allogenic cells, it could be concluded that the antibodies under investigation are autoantibodies. On the basis of physicochemical properties and because of distribution of their reactive antigens, the antibodies under study could be distinguished from previously described autoantibodies of NZB mice.

Lysins for L5178Y cells were also shown in BALB/c, DBA/2, and C3H mice. However, in these strains the optimal binding activity was at 37°C rather than at 4°C. The relation of these antibodies to those in NZB mice was not established by this study.

Footnotes

¹ Supported by United States Public Health Service Research Grant AM-17317 from The National Institute of Arthritis, Metabolism and Digestive Diseases and by The Arthritis Foundation.

² Fellow of Deutsche Forschungsgemeinschaft and Paul Martini Stiftung. Present address: Medizinische Universitaetsklinik, 87 Wuerzburg, Germany.