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Specificity of Autoantibodies to Tubular and Glomerular Basement Membranes Induced in Guinea Pigs¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, California 92037

Abstract

All twenty-eight guinea pigs immunized with a variety of bovine renal basement membrane (RBM) preparations developed similar interstitial nephritides, with minimal glomerular abnormalities. Direct immunofluorescence revealed linear deposition of IgG along the tubular basement membranes (TBM) of all guinea pigs and along the glomerular basement membranes (GBM) of 13 of them. Elution of renal-bound IgG demonstrated that all guinea pigs had antibodies against both TBM and GBM, although anti-GBM activity was usually not detectable at low concentrations of IgG in the eluates. Absorption of pooled eluates with a variety of guinea pig RBM immunoabsorbents revealed the following: 1) approximately three-fourths of the eluted IgG comprised antibody to RBM; 2) all of the eluted autoantibody reacted with TBM, while only 36 to 46% crossreacted with GBM; 3) TBM appeared to have antigenicities both separate from and shared with GBM; 4) guinea pigs immunized with whole bovine TBM appeared to form antibodies to both the collagenous and noncollagenous portions of RBM.

Footnotes

¹ This is Publication No. 719 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation. The work was supported by United States Public Health Service Contract PH-43-68-621 and United States Public Health Service Grant AI-07007. This work was presented in part at the Annual Meetings of the American Society of Experimental Pathologists, Atlantic City, New Jersey, April; 1973 (Lehman, D. H., and Wilson, C. B., Fed. Proc., 32: 3434, 1973).

² Postdoctoral Fellow, Arthritis Foundation.

³ Supported by a fellowship from the Kidney Foundation of Southern California.

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