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Rosette Formation by Guinea Pig Thymocytes and Thymus Derived Lymphocytes with Rabbit Red Blood Cells¹

From the Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111

Abstract

Rabbit red blood cells (RRBC) can bind to guinea pig lymphocytes forming rosettes. Ninety-five per cent of thymocytes, about 50% of lymph node cells, 15% of spleen cells, 30% of peripheral blood lymphocytes and <1% of L₂C (B cell) leukemic lymphocytes form rosettes. Lymphocytes with binding sites for complement (EAC rosetting cells) do not rosette with RRBC. Cells sensitive to the cytotoxic activity of anti-thymus derived (T) cell sera plus complement are the same cells that form RRBC rosettes. Anti-T cell sera (even in the absence of complement) inhibit RRBC rosetting. Rosette formation requires a live lymphocyte and is blocked by sodium azide, but not EDTA. It is concluded that the ability to bind to RRBC is a characteristic of living guinea pig T cells and not of B cells.

Footnotes

¹ This work was supported, in part, by a grant from The American Cancer Society (Massachusetts Division) and by National Institutes of Health Grant AI00436.