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Isolation of Mucin from Human Submaxillary Secretions^1,2,

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Departments of Pediatrics and Biochemistry, University of Florida College of Medicine, Gainesville, Florida 32601

Abstract

Human submaxillary mucin isolated by precipitation with cetyltrimethylammonium bromide dissolved in 3 M sodium chloride was subjected to gel filtration on Sephadex G-200. This glycoprotein was free of contaminating proteins as determined by gel filtration, disc electrophoresis, and immunologic studies. The molecular weight was between 500,000 and 1,000,000. The amino acid composition was similar to submaxillary mucins from other species; however, the carbohydrate composition of human mucins varied significantly from different individuals. The mucin from secretor, blood group A individuals is completely absorbed by columns of anti-A antibody coupled with Sepharose 4B, indicating that the submaxillary mucin and the blood group substances in saliva are identical.

Footnotes

¹ Supported by grants from the National Cystic Fibrosis Research Foundation and the National Institutes of Health.

² A major portion of this work was completed when M. M. Baig was associated with Dr. R. J. Winzler. This paper is dedicated to Dr. Winzler by whose death M. M. Baig lost an excellent teacher and a good friend.

³ Requests for reprints should be sent to: Dr. Baig, Department of Pediatrics, University of Florida College of Medicine, Gainesville, Florida 32610.

⁴ Departments of Pediatrics and Biochemistry, University of Florida College of Medicine, Gainesville, Florida 32610.

⁵ Department of Chemistry, Florida State University, Tallahassee, Florida; deceased, September 28, 1972.