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Expression of HL-A Antigens on Cultured Human Fibroblasts Infected with Mycoplasmas¹

From the Departments of Medical Microbiology and Medicine, Stanford University School of Medicine, Stanford, California 94305, and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

A variety of cultured human fibroblast strains with different HL-A phenotypes were infected with non-fermentative and fermentative mycoplasmas. Both short-term and long-term infections were studied. It was found that many more mycoplasmas associated with the cell membranes of long-term infected cultures than of short-term infected ones. Mycoplasma effects on the morphology of long-term infected cultures varied from an increase in cytoplasmic granularity to frank cytopathogenicity. Regardless of the numbers of mycoplasmas associated with the cells, or the condition of the cells, there was no detectable change in the expression of HL-A antigens measured both qualitatively and quantitatively. It is suggested that HL-A antigens are an essential structural feature of the cytoarchitecture of the cells and/or play a role in transport processes and contact phenomena which are crucial to cell survival.

Footnotes

¹ This study was supported, in part, by National Institutes of Health Research Grants HD04004, HE03365 and AI-10180 from the Institutes of Child Health and Human Development, National Heart Lung, and Allergy and Infectious Diseases; Grant 70-615 from the American Heart Association, Inc., and Grant IC-5 from the American Cancer Society.

² Department of Medical Microbiology, Stanford University School of Medicine, Stanford, California 94305.

³ Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037.

⁴ Department of Medicine, Stanford University School of Medicine, Stanford, California 94305.

⁵ Please send reprint requests to: Dr. Leonard Hayflick, Department of Medical Microbiology, Stanford University School of Medicine, Stanford, California 94305.