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Binding of Maclura Pomifera Lectin to Rat Lymphoid Cells and Erythrocytes¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, California 92037

Abstract

Extracts of Maclura pomifera seeds (MP-Ext), which agglutinate leukocytes and erythrocytes of rats and other animals, were labeled with ¹²⁵I. Labeled extracts were adsorbed to rat cells and eluted (¹²⁵I-MP) by an excess of D-melibiose, a known inhibitor of the binding of seed extracts. Over 80% of ¹²⁵I-MP bound to rat cells. Binding was inhibited by carbohydrates related structurally to D-melibiose but not by lactose, glucose, or carbohydrates unrelated to D-melibiose. Thymus cells bound larger quantities of ¹²⁵I-MP than cells of spleen, bone marrow, or erythrocytes and binding by spleen cells from thymectomized animals was reduced. Thymus cells were also selectively stained by fluor-labeled MP-Ext. ¹²⁵I-MP, phytohemagglutin, and concanavalin A (Con A) were found to recognize distinct receptors on BN rat cells since they did not compete in binding tests and since they are inhibited by different carbohydrates. Eluted MP was mitogenic for spleen cells in serum-free medium, but not in medium containing serum. In addition, it augmented the mitogenic effects of Con A.

Footnotes

¹ This is Publication No. 708 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037. The work was supported by United States Public Health Service Grant AI-07007 and Atomic Energy Commission Contract AT(04-3)-779.