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The Journal of Immunology, 1973, 111: 1690-1698.
Copyright © 1973 by The American Association of Immunologists, Inc.
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Studies of Biologic and Serologic Activities of Rabbit-IgG Antibody Depleted of Carbohydrate Residues1

Ralph C. Williams, Jr., C. Kirk Osterland2, S. Margherita, Sei Tokuda and Ronald P. Messner

From the Department of Medicine, Bernalillo County Medical Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87106, and the Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Abstract

Rabbit IgG preparations containing antibody activity against individual strains of Staphylococcus aureus, Escherichia coli, group A beta-hemolytic streptococci, and pneumococci were digested with Diplococcus pneumoniae endoglycosidase enzyme with removal of more than half of Ig-associated carbohydrate. In addition specifically purified rabbit anti-bovine serum albumin (BSA) IgG was similarly treated. After carbohydrate removal no appreciable change in opsonic, complement fixing, or agglutinating activity was noted in anti-staphylococcal, anti-E. coli, or anti-streptococcal IgG preparations, whereas complete loss of both complement fixing and opsonic activity occurred with rabbit IgG containing anti-pneumococcal antibody activity. No change in passive cutaneous anaphylactic reactions was noted after carbohydrate depletion of immunoabsorbent isolated rabbit anti-BSA IgG antibody. These findings indicate heterogeneous influences of carbohydrate residues on biologic activities of various antibody populations.

Footnotes

1 This work was supported in part by Grants AM13690-03, AM 5548, AM 08490, and AMAI 13789-02 from the United States Public Health Service.

2 Recipient of Senior Faculty Award, American Cancer Society.






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