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The Journal of Immunology, 1973, 111: 1526-1537.
Copyright © 1973 by The American Association of Immunologists, Inc.
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Immune Responses in Vitro

IX. Role of Cell Clusters1

John A. McIntyre2 and Carl W. Pierce3

From the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Abstract

At various intervals after initiation of Mishell-Dutton spleen cell cultures, cell clusters were separated from non-clustered, single spleen cells by sedimentation through a fetal calf serum gradient at unit gravity. Cell clusters collected at all intervals during the initial 48 hr of culture failed to develop subsequently significant plaque-forming cell (PFC) responses. In contrast, the single, non-clustered spleen cells isolated at these intervals subsequently developed PFC responses equal to or greater than responses in unseparated control cultures. Sequential removal of the cell clusters which reformed when the single spleen cells were returned to culture had little or no effect on the PFC responses generated by the remaining single cells. Thus, it appears that the continuous integrity of cell clusters which form in mouse spleen cell cultures has no direct effect on the immune responses which develop in these cultures.

Footnotes

1 This investigation was supported by United States Public Health Service Research Grant AI-09897 from the National Institute of Allergy and Infectious Diseases.

2 Supported by Training Grant GM-02135 from the National Institutes of Health.

3 Recipient of United States Public Health Service Research Career Development Award 1K4-AI-70, 173 from the National Institute of Allergy and Infectious Diseases.






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