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Differentiation of Lymphoid Cells: Effect of Serum and Other Mitogenic Agents on the Selective Induction of Immunoglobulin M Secreting Lymph Node Cells in Tissue Culture

From the National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Rabbit lymph node cells incubated in a serum-free medium exhibit a relatively low level of incorporation of [³H]thymidine into DNA. Fetal calf serum stimulated this incorporation as much as 65-fold. On the other hand, rabbit serum from the same animal that was the source of the cells did not effectively support [³H]thymidine incorporation (only a 0- to 2.7-fold greater incorporation than serum-free medium) and such autologous serum was inhibitory when added in conjunction with fetal calf serum. This same behavior for both fetal calf serum and autologous serum was observed when the emergence of enhanced immunoglobulin M production was assessed rather than [³H]thymidine incorporation.

The emergence of enhanced immunoglobulin M production was markedly inhibited when either concanavalin A, phytohemagglutinin, or pokeweed mitogen was incubated with such cells in tissue culture. In contrast, bacterial endotoxin increased immunoglobulin M production about 2- to 4-fold. Concanavalin A did not inhibit the secretion of immunoglobulin M when added to cells which already exhibit enhanced immunoglobulin M production, i.e., at 48 hr in tissue culture. However, if concanavalin A was added at 24 hr or earlier its full inhibitory activity was manifested.