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The Journal of Immunology, 1973, 111: 1236-1242.
Copyright © 1973 by The American Association of Immunologists, Inc.
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Immunoglobulin Biosynthesis by Normal and Leukemic Human Peripheral Blood Lymphocytes1

Kenneth M. Nies2,3,, M. Anne Oberlin4,5,, John C. Brown3 and Michael S. Halpern5,6,

From the Rheumatic Disease and Immunology Division, Department of Medicine, Los Angeles County/University of Southern California Medical Center, Los Angeles, California 90033 and the Department of Microbiology, University of Southern California, School of Medicine, Los Angeles, California 90033

Abstract

Short-term tissue cultures of peripheral blood lymphocytes isolated from normal individuals and patients with chronic lymphocytic leukemia (CLL) were endogenously labeled with radioactive amino acid and the intracellular and extracellular immunoglobulin isolated by immune precipitation. Even though individual cells from occasional CLL cultures expressed both {gamma}- and µ-chains on their surface as determined by immunofluorescence, each culture in all cases studied synthesized either IgG or IgM but not both. By contrast, cultures of normal peripheral-blood lymphocytes synthesized both IgG and IgM. All normal cultures and certain CLL cultures released labeled immunoglobulin into supernatant fluids.

Footnotes

1 This investigation was supported by Grant AI-10,809-01 from the National Institute of Allergy and Infectious Diseases and the Robert E. and May R. Wright Foundation.

2 Supported by the Arthritis Foundation and Grant AM 05483-07 from the National Institutes of Health.

3 Rheumatic Disease and Immunology Division, Department of Medicine, Los Angeles County/University of Southern California Medical Center, Los Angeles, California 90033.

4 Recipient of National Institute of Allergy and Infectious Diseases Training Grant 5 TO1 AI00157-13.

5 Department of Microbiology, University of Southern California, School of Medicine, Los Angeles, California 90033.

6 Present address: The Wistar Institute, Philadelphia, Pa. 19104.






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