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Production of Functional T Cells after Treatment of Bone Marrow with Thymic Factor¹

From the Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan, 48823 and School of Medicine, Tufts University, Medford, Massachusetts 02160

Abstract

Incubation of bone marrow cells with thymic factor obtained from bovine thymus tissue resulted in the development of cells with T lymphocyte-like function. Adoptive transfer of these cells into lethally irradiated syngeneic mice followed by antigen stimulation with sheep erythrocytes provided a system for testing the ability of the thymic factor-treated cells to induce B cells to become antibody-forming cells. The former were capable of substituting for thymocytes since antibody-forming cells were detected in the recipient spleens upon assay for hemolytic plaques 9 days after transplantation. Limiting dilution experiments in which graded numbers of thymic factor-treated marrow cells were transferred along with a nonrestricted supply of normal bone marrow cells and antigen indicated that the critical number of thymic factor-treated marrow cells needed to synergize with B cells in the production of an anti-SRBC response was contained in 3 x 10⁵ marrow cells. Quantitative and qualitative differences between the thymic factor-treated cells and thymus-derived T cells are discussed.

Footnotes

¹ This work was supported by Grant CA-13396-01 from the National Cancer Institute, National Institutes of Health, United States Public Health Service. Journal Article No. 6243 from the Michigan Agricultural Experiment Station.