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Extraction of Complement Inhibitory Factors from the Erythrocytes of Non-Human Species^1,2,

From the Department of Microbiology, College of Arts and Sciences, and the Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32601

Abstract

Extracts from the erythrocyte stromata of seven species were examined for the presence of a factor capable of accelerating the rate of decay of sheep erythrocytes in the intermediate state
.³ Only the extracts from rabbit and guinea pig erythrocytes possessed decay accelerating activity. Gel filtration (Sephadex G-200) of the active crude extracts gave partial purification with the active fraction passing through the column near the void volume. The materials from rabbit and guinea pig red cells were capable of decreasing the lytic susceptibility of sheep E, EA, EAC_{G P}, EAC4_{G P} and EAC_{G P}4 as well as sheep
. Diluted rabbit and guinea pig erythrocyte stromata extracts, which were incapable of affecting any component of the sheep EAC_{G P}4_{G P} complex, were still capable of accelerating the normal rate of decay of sheep
.

Footnotes

¹ This work was supported by United States Public Health Service Grant AM14244 and the Institute of Food and Agricultural Sciences.

² Authorized for publication as paper number 4766 in the Journal Series of the Florida Agricultural Experiment Station.

³ The nomenclature of complement used conforms to that proposed as a result of a series of World Health Organization meetings (Immunochemistry, 7: 137, 1970). The abbreviations used here are: E, erythrocyte; S, single site of complement activation; A, antibody; C, complement; C1, C2—C9, complement components. Horizontal bars over complement components indicate that the components in question are in biologically active states. Subscripts after complement components identify the species from which the component was derived. For example EAC1_GP are sheep erythrocytes that have been reacted with specific antibody and the first component of guinea pig complement. The overbar indicates that the first component of complement is in the activated state and it possesses esterase activity.

DAF, decay-accelerating factor; SVB, sucrose Veronal buffer; EDTA-VB, ethylenediamine tetraacetate VB; PNS, phosphate-buffered sodium chloride.