HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Philpott, G. W. Articles by Parker, C. W. Search for Related Content PubMed Articles by Philpott, G. W. Articles by Parker, C. W.

Selective Cytotoxicity in a Hapten Substituted Cell Culture Model System¹

From the Departments of Surgery and Medicine, Washington University Medical School, St. Louis, Missouri 63110

Abstract

Selective complement dependent cytotoxicity was demonstrated in a hapten-substituted cell culture model system. Viable hapten substituted HeLa, Hep-2, and L cells were obtained by treatment with 10 to 300 µg/ml of 2, 4, 6-trinitrophenyl (TNP)-sulfonic acid. Binding studies with ¹⁴C-TNP-sulfonic acid showed that from 1 to 9 x 10⁹ TNP groups were bound per cell, representing 1 to 4% of the added TNP. High affinity anti-TNP antibody was purified from the sera of rabbits hyperimmunized with TNP-bovine -globulin. The amount of anti-TNP Ab binding to TNP-cells was quantitated and found to be dose dependent in this range of TNP substitution. Even at the highest levels of antibody, only about 1% of TNP groups was bound by antibody. The selectivity of antibody binding to TNP-cells was established by inhibition with the hapten, -DNP-lysine. Anti-TNP antisera, and to a lesser extent, specifically purified anti-TNP antibody, caused complement dependent cytotoxicity of TNP-cells but not unsubstituted cells. This model system permits comparative cytotoxic antibody studies with a variety of tumor cells.

Footnotes

¹ This work was supported by National Institutes of Health Grant 5R01 CA 12626.

² Address correspondence to: Gordon W. Philpott, M.D., Department of Surgery, Washington University Medical School, 4960 Audubon Avenue, St. Louis, Missouri 63110.

This article has been cited by other articles:

				W. T. Shearer, G. W. Philpott, and C. W. Parker Stimulation of Cells by Antibody Science, December 28, 1973; 182(4119): 1357 - 1359. [Abstract] [PDF]