HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Philpott, G. W. Articles by Parker, C. W. Search for Related Content PubMed Articles by Philpott, G. W. Articles by Parker, C. W.

Selective Cytotoxicity of Hapten-Substituted Cells with an Antibody-Enzyme Conjugate¹

From the Departments of Surgery and Medicine, Washington University Medical School, St. Louis, Missouri 63110

Abstract

Affinity iodination and cytotoxicity of hapten-substituted tumor cells were accomplished with an anti-hapten antibody conjugated to glucose oxidase (which generates H₂O₂) and lactoperoxidase (which iodinates cells in the presence of H₂O₂ and I^-). Polynitrophenylated HeLa and Hep-2 cells, obtained by brief incubation with 2,4,6, trinitrophenyl (TNP)-sulfonic acid, were selectively iodinated and killed when treated briefly with an anti-TNP antibody-glucose oxidase conjugate (Ab-GO) and cultured in media containing lactoperoxidase (LP) and iodide. Cell killing was determined by three methods: 1) a microcytotoxicity assay, 2) the loss of cells prelabeled with ¹²⁵IUdR and 3) the degree of incorporation of ¹²⁵IUdR after treatment (postlabeling). Results with the three independent methods showed cytotoxicity, ranging from 19% to 98% in TNP-cells incubated with Ab-GO (0.6 to 57 µg/ml), LP (50 µg/ml), and iodide (20 µM). -DNP-lysine (100 µM) significantly reduced the amount of killing and minimal cytotoxicity (0 to 35%) was seen with unsubstituted cells or if Ab-GO, LP, or I^- were omitted.

Footnotes

¹ This work was supported by NCI Grants 5R01 CA12626 and AI00219.

² Address Correspondence to: Gordon W. Philpott, M.D., Department of Surgery, Washington University Medical School, 4960 Audubon Avenue, St. Louis, Mo. 63110.

³ W. T. S. is United States Public Health Service Special Research Fellow from the National Institute of Allergy and Infectious Diseases, 1-F03-AI 53856-01.

This article has been cited by other articles:

				M. Rooseboom, J. N. M. Commandeur, and N. P. E. Vermeulen Enzyme-Catalyzed Activation of Anticancer Prodrugs Pharmacol. Rev., March 1, 2004; 56(1): 53 - 102. [Abstract] [Full Text] [PDF]

				W. T. Shearer, G. W. Philpott, and C. W. Parker Stimulation of Cells by Antibody Science, December 28, 1973; 182(4119): 1357 - 1359. [Abstract] [PDF]