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Separation of Lymphocytes from Disaggregated Mouse Malignant Neoplasms by Sedimentation in Gradients of Ficoll in Tissue Culture Medium¹

From the Departments of Pathology and Engineering Biophysics, University of Alabama in Birmingham, Birmingham, Alabama 35294

Abstract

A method is reported for separating lymphocytes from solid tumors. After the sequential use of columns of glass beads, velocity (isokinetic) sedimentation, and isopycnic sedimentation, lymphocytes were obtained in 76.3 to 96.8% purity from disaggregated tumors. When isopycnic sedimentation was omitted, lymphocytes were obtained in 58.4 to 95.1% purity.

Footnotes

¹ This work was supported by grants from the National Institutes of Health (CA-12870, CA-13148, and DE-2670) and the American Cancer Society (ET-50).

² Dr. Pretlow is supported by National Institutes of Health Research Career Development Award K4-CA-70584-01.