HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bach, M. K. Articles by Brashler, J. R. Search for Related Content PubMed Articles by Bach, M. K. Articles by Brashler, J. R.

On the Nature of the Presumed Receptor for IgE on Mast Cells

II. Demonstration of the Specific Binding of IgE to Cell-Free Particulate Preparations from Rat Peritoneal Mast Cells

From the Department of Hypersensitivity Diseases Research, The Upjohn Company, Kalamazoo, Michigan 49001

Abstract

The preparation of mixed rat peritoneal cells for antigen-induced release of histamine by IgE-rich antibody preparations is accompanied by the removal of passive cutaneous anaphylaxis-reactive antibodies from the antibody solution. This removal of antibody from the solutions is directly related to the mast cell content of the mixed cell suspension: little antibody is removed when cells from distilled water-treated rats are used, and the amount of antibody removed by isolated mast cells accounts for the antibody-binding activity of mixed cell preparations. Lysis of isolated mast cells in distilled water or their disruption by mild sonication does not destroy their apparent IgE antibody-binding capacity. Frozen, washed particulate preparations from isolated mast cells retain their ability to bind IgE. Binding of IgE is time dependent. The binding is inhibited by IgGa antibody preparations in a manner which is consistent with competitive inhibition.