HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Henney, C. S. Articles by Bubbers, J. E. Search for Related Content PubMed Articles by Henney, C. S. Articles by Bubbers, J. E.

Antigen-T Lymphocyte Interactions: Inhibition by Cytochalasin B^1,2,

From the Department of Medicine of the Johns Hopkins University, School of Medicine and the O'Neill Memorial Research Laboratories of the Good Samaritan Hospital, Baltimore, Maryland

Abstract

The adsorption of cytologically active C₅₇BL/6 T lymphocytes onto homologous DBA/2 embryonic fibroblast monolayers has been studied in the presence of a number of drugs. The adsorption of cytolytic effector cells onto monolayers was unaffected by prostaglandin E₁ or by colchicine, both of which are potent inhibitors of lymphocyte-mediated cytolysis. On the other hand, the specific adsorption of lymphocytes onto the monolayers was markedly inhibited by cytochalasin B. These findings reinforce the concept that T cell-mediated cytolysis proceeds in discrete steps which are inhibitable at different levels. Further, in view of the known effects of cytochalasin B, these studies suggest that membrane modulation is required for T lymphocyte-antigen interactions.

Footnotes

¹ This work was supported by Grant AI 10280 and Research Career Development Award AI 70393 to C. S. H. from the National Institutes of Allergy and Infectious Diseases and a grant from the National Science Foundation. This is Communication #74 from O'Neill Memorial Laboratories.

² Abbreviations used in this paper: T. thymus derived; PGE₁, prostaglandin E₁; PGE₂, prostaglandin E₂; DMSO, dimethylsulfoxide; MES, Eagle's minimal essential medium with 10% inactivated fetal calf serum; HBSS, Hanks' balanced salt solution.