HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Waldron, J. A. Articles by Rosenthal, A. S. Search for Related Content PubMed Articles by Waldron, J. A., Jr. Articles by Rosenthal, A. S.

Antigen-Induced Proliferation of Guinea Pig Lymphocytes in Vitro: Obligatory Role of Macrophages in the Recognition of Antigen by Immune T-Lymphocytes

From the Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014, and the Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Abstract

Highly purified lymphocytes were obtained from the lymph nodes of CFA-immune guinea pigs. Cultures of these lymphocytes did not exhibit proliferative responses upon stimulation with purified protein derivative (PPD), although they were quite responsive to stimulation with phytohemagglutinin. Addition of macrophages to these cultures resulted in marked enhancement of the PPD-induced lymphoproliferative response, but did not affect lymphocyte responsiveness to phytohemagglutinin. The ability of the purified immune lymphocytes to interact directly with soluble PPD was examined by incubating the cells for 1 hr at 37°C with various concentrations of PPD. The lymphocytes were then washed to remove unbound antigen and placed in culture. Addition of macrophages to cultures of antigen-pulsed lymphocytes did not result in the significant DNA synthesis, suggesting that the specifically immune lymphocytes were unable to bind and carry into culture sufficient antigen to allow for their subsequent activation. These results were not due to the induction of tolerance in the lymphocytes, since cultures of antigen-pulsed and untreated lymphocytes were equally responsive to continuous PPD when macrophages were added to the cultures, even when the antigen-pulsed lymphocytes had been exposed to high concentrations of PPD for 1 or 24 hr. In contrast, macrophages which were incubated for 1 hr at 37°C with a given concentration of PPD were as effective as the same concentration of PPD present continuously in macrophage-lymphocyte cultures in the induction of a lymphoproliferative response. Macrophages appear to play an obligatory role in the presentation of antigen to the immunospecific thymus-derived (T)-lymphocyte.

Footnotes

¹ Vivian Allen Medical Scholar, Vanderbilt University School of Medicine. Previously supported by United States Public Health Service Training Grant GM-00290. This work submitted in partial fulfillment of requirements for Ph.D. in Pathology.

² Recipient of United States Public Health Service Career Development Award GM-28110. This investigation was supported in part by United States Public Health Service Grant HE-10048-07.

³ Address reprint requests to Alan S. Rosenthal, M.D., Section on Biologic Structure, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, NIH, Bldg. 10, Room 11N-224, Bethesda, Maryland 20014.

This article has been cited by other articles:

				P. M. Allen Making Antigen Presentable J. Immunol., July 1, 2007; 179(1): 3 - 4. [Full Text] [PDF]