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The Journal of Immunology, 1973, 111: 152-156.
Copyright © 1973 by The American Association of Immunologists, Inc.
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Intracellular Localization of Mammalian Type C Virus Species-Specific (gs-1) and Interspecies-Specific (gs-3) Antigenic Determinants with the Indirect Immunoperoxidase Technique and Light Microscopy1

Anthony Girardi2, Berge Hampar3, Konrad C. Hsu4, Stephan Oroszlan4, Elizabeth Hornberger1, Gary Kelloff3 and Raymond V. Gilden5

From the Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 and Viral Carcinogenesis Branch, National Cancer Institute, Bethesda, Maryland 20014

Abstract

The indirect immunoperoxidase staining technique was used for localizing by light microscopy the intracellular species-specific and interspecies-specific antigenic determinants of a panel of murine and hamster type C viruses, and was compared to the immunofluorescent technique. The antisera were prepared against antigens purified by isoelectric focusing and also were obtained from MSV tumor-bearing rats. The results indicate that the immunoperoxidase technique is suitable for detecting antigens associated with mammalian type C viruses, and appears to be more sensitive than the immunofluorescent procedure without sacrificing specificity.

Footnotes

1 This work was supported in part by Contract NIH-NCI-E-71-29097, and at the Wistar Institute under Contract 71-2092 within the Special Virus Cancer Program of the National Cancer Institute, National Institutes of Health, Public Health Service.

2 The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania.

3 Viral Carcinogenesis Branch, National Cancer Institute.

4 Columbia University, New York, New York.

5 Flow Laboratories, Inc., Rockville, Maryland.






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