The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1973, 111: 144-151.
Copyright © 1973 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sell, S.
Right arrow Articles by Gell, P. G. H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sell, S.
Right arrow Articles by Gell, P. G. H.

Studies on Rabbit Lymphocytes in Vitro

XVI. Restimulation of Antiallotypically Stimulated and Blocked Cultures1

Stewart Sell2, Jennifer A. Lowe and P. G. H. Gell

From the Department of Pathology, School of Medicine, University of California at San Diego, La Jolla, California, and the Department of Experimental Pathology, University of Birmingham Medical School, Birmingham 15, England

Abstract

Rabbit peripheral blood lymphocytes that have been stimulated in vitro to transform into blast cells by antiimmunoglobulin allotypic sera and then blocked by addition of serum containing the allotype with which the stimulating antiserum reacts may be restimulated by subsequent removal of the blocking serum and readdition of the stimulating antiserum. The extent of stimulation produced by stimulation-block-restimulation is the same as that produced by the continuous presence of the antiallotypic serum (continuous stimulation). However, if unstimulated cultures are incubated in vitro in neutral serum before stimulation with antiallotypic serum (delayed stimulation), the extent of stimulation is less than that produced by continuous stimulation or by stimulation-block-restimulation. Prestimulation of double homozygous lymphocytes with antiserum to one allotypic specificity activates the cells to respond to delayed restimulation with antiserum to the other allotypic specificity (mixed allotypic sequential stimulation, MASS). This MASS activation is not observed in cultures of mixtures of lymphocytes with antiallotypic sera to specificities contributed by different donors. These results indicate that continued contact of the transforming cells with the stimulating agent (signal maintenance) is required to consummate the transformation process and that cultures that are stimulated and blocked may be restimulated by a second specific signal.

Footnotes

1 Presented in part at the Federation of American Societies of Experimental Biology meeting in Atlantic City, April 13, 1972. Supported by Grant AI-09719 from National Institutes of Allergy and Infectious Diseases, National Institutes of Health, and by the Medical Research Council.

2 Address correspondence to S. Sell, Department of Pathology, School of Medicine, UCSD, La Jolla, California 92037.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1973 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1973 by The American Association of Immunologists, Inc. All rights reserved.