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Cellular Requirements for Development of Primary Anti-Hapten Antibody Responses in Vitro¹

From The Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Abstract

The cellular requirements for the development of primary trinitrophenyl (TNP)-specific plaque-forming cell (PFC) responses to TNP-X in cultures of spleen cells from normal mice and from mice immunized 7 days earlier with X were compared. Significant enhancement of the primary anti-TNP PFC responses of both the G and M immunoglobulin classes was demonstrated in cultures of X-primed spleen cells stimulated with TNP-X at a concentration suboptimal for normal spleen cells. After separation of normal and X-primed spleen cells into macrophage and lymphoid cell populations on the basis of the ability of macrophages to adhere to plastic, it was demonstrated that primary TNP-specific PFC responses of normal spleen cells were strictly dependent on the presence of the macrophages, while the responses of X-primed spleen cells were much less dependent on their presence.

The depletion of -bearing cells from cultures of normal spleen cells by treatment with anti- serum and complement minimized primary TNP-specific PFC responses to TNP-X. Depletion of the -bearing cells from cultures of spleen cells from X-primed mice reduced primary TNP-specific PFC responses only 10% to 50%, while the primary anti-horse red cell PFC responses in the same cell preparations were reduced over 90%.

Footnotes

¹ This investigation was supported by United States Public Health Service Research Grants AI-09897 and AI-09920 from the National Institute of Allergy and Infectious Diseases.

² Present address: Department of Medicine, University Hospital, University of California, San Diego, La Jolla, California 92037.

³ Recipient of United States Public Health Service Research Career Development Award 1K4-AI-70, 173 from the National Institute of Allergy and Infectious Diseases.

Address correspondence and reprint requests to Carl W. Pierce, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.