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Immune Response of Mice to Nucleic Acids: Strain-Dependent Differences in Magnitude and Class of Antibody Production

From the Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

Abstract

Several inbred mouse strains were immunized with denatured DNA-methylated bovine serum albumin (MBSA) complexes. The response of SJL/J mice was stronger over a period of 53 days (including 3 injections) than that of DBA/2, AKR/Cu, C3H/HeJ, C57BL/6 and BALB/c mice.

In a kinetic study of serum antibody levels, the early (10-day) responses of SJL/J and DBA/2 mice were similar, but the strength of SJL/J sera continued to rise while that of DBA/2 animals fell. After a boost at day 21, the SJL/J mice produced much higher antibody titers than did DBA/2 animals. In parallel assays for direct plaque-forming cells, DBA/2 animals yielded higher number of plaques at day 10, but similar values for both strains were derived at later times, including after the booster injection. Analysis by Sephadex G-200 gel filtration showed that the lower-responding DBA/2 animals produced only 19S antibody, while SJL/J mice produced both 19S and large amounts of 7S antibody.

A reversed pattern was observed after immunization with poly(A)·poly(U) or poly(I)·poly(C). In this case, DBA/2 mice responded more strongly than did SJL/J mice in production of antibodies to the double-helical polyribonucleotides.

Footnotes

¹ On leave from Department of Biochemistry and Pharmacology, Tufts University School of Medicine, Boston, Massachusetts 02111, U.S.A. Recipient of senior fellowship from Weizmann Institute of Science.

² Send correspondence and proofs to: Dr. Sara Fuchs, Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel.

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