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The Use of Column-Bound B Lymphocytes for Absorption of Antisera to T Lymphocyte Antigens¹

From the Department of Immunology, Middlesex Hospital Medical School, 40–50 Tottenham Street, London, W1P 9PG, England

Abstract

Antisera raised in rabbits to BALB/c brain homogenates were not specific for thymus-derived (T) lymphocytes when tested by indirect immunofluorescence. After absorption with liver cells, erythrocytes and bone marrow-derived (B) cells adsorbed to an anti-Fab immunoadsorbent column, the antisera stained all thymocytes from BALB/c and AKR mice but only a proportion of lymphocytes from spleen and lymph node. Virtually no cells were stained in nude mouse lymphocyte suspensions. Staining of lymphocytes fractioned by affinity chromatography into T and B cells provided further evidence for the specificity of the absorbed antiserum.

Footnotes

¹ This work was supported by grants from the Medical Research Council of Great Britain.