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The Journal of Immunology, 1973, 110: 1630-1637.
Copyright © 1973 by The American Association of Immunologists, Inc.
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Guinea Pig Lymphotoxin (LT)

II. Physicochemical Properties of LT Produced by Lymphocytes Stimulated with Antigen or Concanavalin A: Its Differentiation from Migration-Inhibitory Factor (MIF)1

John A. Coyne2, Heinz G. Remold3, Steven A. Rosenberg4 and John R. David

From the Departments of Medicine, Harvard Medical School and the Robert B. Brigham Hospital, Boston, Massachusetts 02120

Abstract

Guinea pig lymphotoxin (LT) produced by stimulation of sensitized lymph node cells, specifically by antigen or non-specifically by concanavalin A, was studied by methods previously used to characterize guinea pig migration-inhibitory factor. The results showed that LT induced by either antigen or mitogen was heat labile, eluted from Sephadex columns with peak activity in fractions containing molecules of 35,000 to 55,000 m.w., had an electrophoretic mobility at pH 9.1 similar to albumin and a buoyant density on CsCl similar to protein. It was destroyed by chymotrypsin but not by neuraminidase. These studies show that guinea pig LT can be readily differentiated from guinea pig migration-inhibitory factor. On the other hand, the LT produced by antigen stimulation has many features in common with LT produced by stimulation with concanavalin A.

Footnotes

1 This work was supported in part by United States Public Health Service Grant AI07685, National Institutes of Health Contract 71-2172, and a grant from the John A. Hartford Foundation.

2 Supported by United States Public Health Service Immunology Training Grant AI0036603.

3 Recipient of Cancer Research Scholar Award from the American Cancer Society.

4 Supported by United States Public Health Service Training Grant, Department of Surgery, Peter B. Brigham Hospital.






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