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Guinea Pig Lymphotoxin (LT)

I. In Vitro Studies of LT Produced in Response to Antigen Stimulation of Lymphocytes¹

From the Department of Medicine, Harvard Medical School, and the Robert B. Brigham Hospital, Boston, Massachusetts 02120

Abstract

Lymphotoxin (LT) was found to be present in the same supernatants as antigen-stimulated guinea pig lymphocytes in which migration-inhibitory factor (MIF) was found. LT activity was determined by staining target cells with trypan blue, counting the cells present in the media and determining their viability, direct observation by time-lapse cinematography and measuring protein synthesis by surviving target cells. Like migration-inhibitory factor, the induction of LT release was antigen specific. LT was effective on a number of different target cell lines; however, young cultures of guinea pig embryo fibroblasts were resistant to it.

Footnotes

¹ This work was supported in part by United States Public Health Service Grant AI07685, National Institutes of Health Contract 71-2172, and a grant from the John A. Hartford Foundation.

² Supported by a United States Public Health Service Training Grant, Department of Surgery, Peter B. Brigham Hospital.

³ Supported by United States Public Health Service Immunology Training Grant AI0036603.