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Lymphocyte Populations: Separation by Discontinuous Density Gradient Centrifugation¹

From the School of Medicine, Departments of Medicine and Surgery, University of California at Los Angeles, Los Angeles, California 90024

Abstract

A method for separation of human lymphocytes by discontinuous density gradient is described. The method was simple to carry out and utilized Ficoll-salt solutions which were easily standardized. There was good reproducibility with no toxicity to the cells. On applying this method to human thoracic duct lymphocytes, cells were separated into subpopulations which differed in morphology, basal unstimulated ³H-TdR incorporation and response to stimulation by purified phytohemagglutinin P and pokeweek mitogen.

Footnotes

¹ This work was supported by National Institutes of Health Grant GM 15759.

² David Tak Yan Yu is a Research Fellow of the Southern California Arthritis Foundation.

³ Requests for reprints should be addressed to Dr. J. B. Peter, Department of Medicine, University of California, Los Angeles, California 90024.